Models of maximum stress and strain of zebrafish embryos under indentation |
| |
Authors: | Zhe Lu Peter CY Chen Hong Luo Joohoo Nam Ruowen Ge Wei Lin |
| |
Institution: | 1. University of Saskatchewan, Department of Soil Science, Saskatoon, SK S7N 5A8, Canada;2. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;3. Laboratory of Riverine Ecological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China |
| |
Abstract: | Micro-injection of zebrafish embryo is widely applied in biology for the analysis of early developmental processes. The success of a micro-injection to a large extent depends on the mechanical interaction between the micro-pipette and the membrane of the zebrafish embryo. In this paper, we present the development of (i) a maximum stress model of the deformed membrane with respect to the depth of indentation, (ii) a family-of-conics elongation model to determine the length of the deformed membrane for the estimation of the maximum strain at a given indentation depth, and (iii) an experimental system to generate the required data for these two models. The significance of these results is that the estimated maximum stress provides a performance target for the penetration process, while the estimated corresponding maximum strain serves as an indicator of the extent of deformation sustained by the embryo prior to penetration. Implications of these modeling and experimental results are discussed in the context of optimizing the process of micro-injection. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|