Topographic mapping and compression elasticity analysis of skinned cardiac muscle fibers in vitro with atomic force microscopy and nanoindentation |
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| Authors: | Jie Zhu Tanya Sabharwal Aruna Kalyanasundaram Lianhong Guo Guodong Wang |
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| Institution: | 1. Cardiac Biophysics and Bioengineering Laboratory, College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China;2. Biophysics Collaborative Access Team, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439-4860, United States;3. Pritzker Institute of Biomedical Science & Engineering, University Technology Park at IIT, Chicago, IL 60616-3793, United States;4. Section of Molecular Cell and Developmental Biology, School of Biological Sciences, University of Texas, Austin, TX 78712, United States;5. Laboratory of Biomathematics, Department of Applied Mathematics, Northwest A&F University, Yangling, Shaanxi 712100, China;6. Department of Applied Mathematics, College of Science and Letters, Illinois Institute of Technology, Chicago, IL 60616, United States;1. Laboratoire Imagerie Moléculaire In Vivo (IMIV), UMR 1023 Inserm/CEA/Université Paris Sud ? ERL 9218 CNRS, CEA/I2BM/SHFJ, Orsay, France;2. Radiology department, Hôpital Pitié-Salpétrière, APHP, Paris, France;3. Radiology department, Hôpital Necker, APHP, Paris, France;4. Sorbonne Universités, UPMC Univ Paris 06, CNRS, INSERM, Laboratoire d’Imagerie Biomédicale (LIB), 75006 (ou 75013), Paris, France;1. Department of Emergency and Organ Transplant, Bari University, Bari, Italy;2. Hedenstierna Laboratoriet—Surgical Sciences, Uppsala University, Uppsala, Sweden;3. Department of Anaesthesia and Intensive Care Medicine, Sahlgrenska University Hospital, Göteborg, Sweden;4. Hedenstierna Laboratoriet—Medical Sciences, Uppsala University, Uppsala, Sweden;1. Faculty of Engineering, Universiti Putra Malaysia, UPM Serdang, 43400 Serdang, Selangor, Malaysia;2. Center of Diagnostic Nuclear Imaging, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia;3. Faculty of Science, Universiti Teknologi Malaysia, UTM Skudai, 81300 Skudai, Johor, Malaysia;1. GEROM Groupe Etudes Remodelage Osseux et bioMatériaux, LUNAM Université, IRIS-IBS Institut de Biologie en Santé, CHU d‘Angers, 49933 Angers Cedex, France;2. UPSP “Biologie et Biomatériaux du Tissus Osseux – Chirurgie Expérimentale”, ONIRIS – National Veterinary School of Nantes, LUNAM Université, Route de Gâchet, BP 44706, F44307 Nantes cedex 03, France;3. Service de Rhumatologie, CHU d‘Angers, 49933 Angers Cedex, France;1. Centre for Molecular Imaging, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia;2. Department of Physical Sciences, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia;3. Department of Radiation Oncology, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia;4. Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, Australia;5. Department of Applied Physics, RMIT University, Melbourne, Australia |
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| Abstract: | Surface topography and compression elasticity of bovine cardiac muscle fibers in rigor and relaxing state have been studied with atomic force microscopy. Characteristic sarcomere patterns running along the longitudinal axis of the fibers were clearly observed, and Z-lines, M-lines, I-bands, and A-bands can be distinguished through comparing with TEM images and force curves. AFM height images of fibers had shown a sarcomere length of 1.22±0.02 μm (n=5) in rigor with a significant 9% increase in sarcomere length in relaxing state (1.33±0.03 μm, n=5), indicating that overlap moves with the changing physiological conditions. Compression elasticity curves along with sarcomere locations have been taken by AFM compression processing. Coefficient of Z-line, I-band, Overlap, and M-line are 25±2, 8±1, 10±1, and 17±1.5 pN/nm respectively in rigor state, and 18±2.5, 4±0.5, 6±1, and 11±0.5 pN/nm respectively in relaxing state. Young's Modulus in Z-line, I-band, Overlap, and M-line are 115±12, 48±9, 52±8, and 90±12 kPa respectively in rigor, and 98±10, 23±4, 42±4, and 65±7 kPa respectively in relaxing state. The elasticity curves have shown a similar appearance to the section analysis profile of AFM height images of sarcomere and the distance between adjacent largest coefficient and Young's Modulus is equal to the sarcomere length measured from the AFM height images using section analysis, indicating that mechanic properties of fibers have a similar periodicity to the topography of fibers. |
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