重组别藻蓝蛋(rAPC)抑瘤活性及其对免疫作用的研究

目的:观察重组别藻蓝蛋白(rAPC)对接种H22肝癌细胞小鼠的抑瘤活性及其免疫作用.方法:昆明小鼠随机分为5组(10只/组),即模型组、rAPC低、中、高剂量组(25,50,100mg/kg·d)、环磷酰胺组(CY对照组).建立小鼠肝癌1422移植瘤模型,次日除模型组外,其余4组分别以不同剂量的rAPe、CY灌胃,于15d后处死,完整剥离出肿瘤、胸腺、脾脏,准确称重,计算抑瘤率、胸腺指数和脾指数,并采用放免法(PIA)测定血清中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的水平.结果:rAPC低剂量组小鼠H22肿瘤质量增长较模型组缓慢(P＜0.05),中、高剂量组较模型组显著缓慢(P＜0.01),抑瘤率分别为25.2%、36.7%、43.1%;rAPC各剂量组能升高胸腺指数、脾指数和血清中细胞因子IL-6、TNF-α的水平.结论:rAPC可有效抑制H22肝癌的生长,促进小鼠胸腺和脾脏的生长发育,提高小鼠的免疫功能,从而抑制肿瘤的生长.

Anticancer activities and immunologic functions of rAPC

Objective:To investigate the anticancer and immunologic activities of recombinant Allophycocyanin(rAPC)in H22 mice.Methods:KM mice were randomly divided into five groups:the model group,the rAPC groups(25,50,100mg/kg·d),the Cyclophosphamide group(the control group)and each group had ten mice.Transplant tumor model of implanted hepatoma 22(H22)was established.Four groups of animals(except the model group)were treated with different dosage of rAPC(ig)and CY(ip)from the second day and executed after 15 days.Tumors,thymuses and spleens were weighed and tumor inhibition ratio,index of thymus and spleen were detected.Furthermore the level of IL-6 and TNF-α in serum were determined by RIA.Results:In vivo,the increase of H22 tumor weight in rAPC low-dose group was slower than that in model group(P＜0.05),while the weight of tumor in mid and high-dose groups was inhibited obviously(P＜0.01).The tumor inhibition rate was 25.2%,36.7%,43.1% respectively.The rAPC can obviously increase the level of index of thymus and spleen and the level of IL-6 and TNF-α in serum.Conclusion:rAPC showed obvious anticancer activities and immunologic functions.