An immunocytochemical method for assaying oestrogen receptors in breast cancers. A comparison with the steroid binding assay |
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Authors: | L O Ottestad K M Tveit J M Nesland I Dybsjord O B?rmer J H?ie A Pihl |
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Affiliation: | Department of Biochemistry, Norwegian Radium Hospital, Oslo. |
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Abstract: | The presence of oestrogen receptors was studied in 105 human breast carcinomas using monoclonal antibodies (Abbott ER-ICA kit). The oestrogen receptors of neoplastic cells were semiquantitatively measured and correlations were made to receptor values determined by a dextran-coated charcoal (DCC) steroid binding assay and to histological grade. Immunoreactive cells were found in about 2/3 of the tumours. Usually only a fraction of the cells within each tumour were immunoreactive, and the staining intensity varied among different cells. In general, well differentiated tumours had a greater proportion of immunoreactive cells than poorly differentiated ones. In most cases (65/98) a good agreement was found between the ER-ICA method and the DCC assay. However, in 33 cases discrepancies were demonstrated. |
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