Osmotic regulation of ATA2 mRNA expression and amino acid transport System A activity |
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Authors: | Alfieri R R Petronini P G Bonelli M A Caccamo A E Cavazzoni A Borghetti A F Wheeler K P |
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Institution: | Dipartimento di Medicina Sperimentale, Sezione di Patologia Molecolare e Immunologia, Università degli Studi di Parma, Parma, 43100, Italy. |
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Abstract: | When porcine endothelial cells were exposed to hypertonicity, both the level of ATA2 (amino acid transporter 2) mRNA and activity of amino acid transport System A increased transiently, peaking after about 6 and 9 h, respectively. Cycloheximide, like actinomycin D, prevented both responses, showing that an earlier step also involves protein synthesis. Withdrawal of hypertonicity after 6 h increased the rate of down regulation. These findings confirm that ATA2 is a major isoform of System A and show that changes in the expression of ATA2 mRNA precede both the induction and subsequent down regulation of transport activity. |
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