TTC4,a Novel Human Gene Containing the Tetratricopeptide Repeat and Mapping to the Region of Chromosome 1p31 That Is Frequently Deleted in Sporadic Breast Cancer |
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| Affiliation: | 1. Department of Operation, The Affiliated Hospital of Southwest Medical University, Luzhou, China;2. School of Pubilc Health, Guangdong Pharmaceutical University, Guangzhou, China;3. People''s Hospital of Luxian, Luzhou, China;4. Department of Urology, Chengdu Chengfei Hospital, Chengdu, China;5. Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, China;1. Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi Arabia;2. Sciences Faculty for Girls, King Abdulaziz University, Jeddah 21589, Saudi Arabia;3. Inter-University Accelerator Centre, Aruna Asaf Ali Marg, New Delhi 110067, India;1. Department of Oncology, Cancer Medical Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu, P.R. China;2. Department of Epidemiology and Biostatistics, Center for Global Health, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, School of Public Health, Nanjing Medical University, Nanjing 211100, P.R. China |
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| Abstract: | The 1p31 region shows loss of heterozygosity in up to 50% of human breast cancers, indicating the presence of a tumor suppressor gene in this location. We have mapped six novel ESTs to a 15-Mb contig of yeast artificial chromosomes spanning the critical region of 1p31. One of these ESTs was localized within the contig to the region most commonly undergoing loss of heterozygosity in breast cancer. The corresponding gene sequence for this EST was established by cDNA cloning and RACE procedures. This gene is 2 kb long and contains a tetratricopeptide repeat motif and a coiled-coil domain. This family of genes has been implicated in a wide variety of functions, including tumorigenesis. This is the fourth member of the human gene family, and so we have named this geneTTC4.Northern blot analysis demonstrates a ubiquitous pattern of gene expression that includes breast tissue. A preliminary screen of human breast cancer cell lines shows thatTTC4is expressed in all cases, but SSCP analysis of the coding region of this gene following RT-PCR failed to reveal any mutations. Clearly, because of its map location, a more extensive analysis is warranted to establish whether subtle mutations are present in breast cancers. |
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