MouseSprr2Genes: A Clustered Family of Genes Showing Differential Expression in Epithelial Tissues |
| |
| Affiliation: | 1. Department of Ophthalmology, Shanghai First People''s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China;2. Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai First People''s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China;3. Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;4. School of Life Sciences, Shanghai University, Shanghai 200444, China;5. Department of Chemistry, Shanghai University, Shanghai 200072, China |
| |
| Abstract: | Small proline-rich (SPR) proteins are structural components of the cornified cell envelope of stratified squamous epithelia. They are subdivided into three families, i.e., SPR1, SPR2, and SPR3, of which the SPR2 family is the most complex. To understand the significance of this complexity, we have isolated 11 mouseSprr2genes, constructed a provisional physical map of theSprr2locus on mouse Chromosome 3, and examined the expression patterns of theSprr2genes in mouse epithelial tissues. The 11Sprr2sequences are highly conserved with a central domain containing a variable number of repeats.In situhybridization showed theSprr2expression to be confined to epithelia. RT-PCR using primers specific for each of the 11Sprr2members demonstrated varying degrees of expression among the individualSprr2members in different tissues. The correlation between the physical location of the genes in theSprr2locus and their expression patterns suggests multiple levels of controlled expression. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
