福建橄榄18S-26S rRNA及其ITS片段的克隆与序列分析

利用18S-26S rRNA基因及其ITS片段的PCR扩增、克隆及测序分析,对福建14个橄榄品种进行分子标记及遗传分类。序列分析结果表明:14个品种可分为4个类别,以1号品种为参照,3、4、6、7、8、9、11号品种的ITS1、ITS2和5.8S共634个碱基序列完全一致,与1号仅ITS2上有1个碱基差异,2号和1号品种的ITS1和5.8S序列完全一致,仅ITS2上有2个碱基差异,归为第一类;5号和14号的序列完全一致,与1号在ITS1和ITS2上各有1个碱基差异,归为第二类;10号和12号序列完全一致,与1号在ITS1上有2个碱基差异,归为第三类;13号品种5.8S的序列与1号相同,但在ITS1上有1个碱基差异,在ITS2上有24个碱基差异,归为第四类。使用DNAMAN软件建立的同源关系树说明了不同橄榄品种的变异程度。将橄榄18S-26S rRNA基因及其ITS序列登陆GenBank,登陆号:DQ517524。

Cloning and Sequencing of 18S-26S rRNA and ITS on Canarium album in Fujian Province

Cloning and sequencing were applied to analyze 18S-26S rRNA and ITS on 14 varieties of Canarium album.The results showed that the 14 varieties of Canarium album could be partitioned to 4 groups.Taking No.1 variety as control sample,the sequence of total 634 nucleotides in ITS1,ITS2 and 5.8S was same on No.3,4,6,7,8,9,11 variety,and only one nucleotide differed from the control in ITS2,and No.2 variety had two nucleotides differed from the control in ITS2,and the 9 varieties above belonged to the first group.The sequences of No.5 and No.14 were same,and had one nucleotide difference in ITS1 and ITS2 respectively compared to the control,No.5 and 14 varieties belonged to the second group.The sequences of No.10 and No.12 were same,and had two different nucleotides in ITS1 compared to the control,and No.10 and No.12 varieties belonged to the third group.The last group was No.13 variety,which had one and twenty-four different nucleotides in ITS1 and ITS2 respectively compared to the control.The homology tree established by DNAMAN showed the variation among the 14 varieties of Canarium album.And the sequence of Canarium album deposited in GenBank under number DQ517524.