Effect of bacitracin on the biodegradation of β-endorphin |
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Authors: | András Patthy László Gráf Ágnes Kenessey József I. Székely Sándor Bajusz |
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Affiliation: | Research Institute for Pharmaceutical Chemistry, H-1325 Budapest, P.O. Box 82, Hungary |
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Abstract: | β-endorphin was incubated with rat brain homogenate, and the amino acids released were measured by amino acid analysis. Phe, Leu, Tyr, and Lys were liberated in the greatest amount indicating that the cleavage of Leu77-Phe78 and some Lys-X peptide bonds with endopeptidases followed by the removal of the terminal residues by exopeptidases are the main routes of β-endorphin degradation in the brain. Bacitracin considerably reduced the amino acid release from β-endorphin incubated with rat brain homogenate, and its action is suggested to be due to the inhibition of brain amino- and carboxypeptidases. Bacitracin also potentiated and prolonged the analgesic activity of β-endorphin. |
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Keywords: | To whom correspondance should be addressed. |
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