Self redigestion of native chromatin |
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| Authors: | C.W. Carter L.F. Levinger |
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| Affiliation: | Departments of Biochemistry and Anatomy, The University of North Carolina at Chapel Hill, Swing Building 217-H, Chapel Hill, N.C. 27514 USA |
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| Abstract: | Chromatin prepared by micrococcal nuclease digestion of nuclei and fractionated by sucrose density gradient ultracentrifugation selfredigests to a higher percentage of monomer DNA at a rate which depends on the enzyme concentration in the initial digestion. Thus, micrococcal nuclease becomes part of the native chromatin structure. A degree of caution should therefore be exercised when handling material prepared by this technique. |
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