Singlet oxygen quenchers and the photodynamic inactivation of E. coli ribosomes by methylene blue |
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Authors: | Harwant Singh Joseph A Vadasz |
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Institution: | Medical Biophysics Branch, Atomic Energy of Canada Limited, Whiteshell Nuclear Research Establishment, Pinawa, Manitoba, Canada ROE 1LO |
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Abstract: | ribosomes are readily photoinactivated by methylene blue in the presence of air. A variety of singlet oxygen quenchers like NaN3, 2,5-dimethylfuran, hydroquinone and ascorbic acid provide about 60% protection against this photoinactivation indicating that a major mechanism of ribosome inactivation proceeds through the formation of singlet oxygen, with small contributions (<40%) from other mechanisms. The singlet oxygen quenchers, 1,4-diazabicyclo 2.2.2] octane and triethylamine give unexpected results, in that they show no protection against photoinactivation. |
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Keywords: | DABCO 1 4-diazabicyclo [2 2 2] octane singlet oxygen superoxide anion |
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