Platelet plasma membrane glycoproteins Identification of a proteolytic substrate for thrombin |
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Authors: | David R. Phillips Patricia Poh Agin |
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Affiliation: | Department of Biochemistry St. Jude Children''s Research Hospital 332 North Lauderdale Memphis, Tennessee 38101 USA |
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Abstract: | The surface glycoproteins of the platelet plasma membrane were labeled by oxidation with galactose oxidase followed by reduction with (3H)-sodium borohydride. Of the glycoproteins labeled, only glycoprotein V (apparent molecular weight of 89,000) was decreased as a result of thrombin action. The affected glycoprotein appeared to be completely removed at a concentration of 1 U thrombin per 109 platelets. A soluble glycopeptide hydrolytic product with an apparent molecular weight of 70,000 was released into solution. |
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