Use of the coulombic interactions of the lanthanide series to identify two classes of Ca2+ binding sites in mitochondria |
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Authors: | William P. Tew |
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Affiliation: | Department of Physiological Chemistry The Johns Hopkins University School of Medicine 725 N. Wolfe Street, Baltimore, MD 21205 USA |
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Abstract: | The degree of inhibition of respiration-dependent vs respiration-independent Ca2+ binding by rat liver mitochondria by different members of the lanthanide family was used to establish the existence of two different classes of Ca2+ binding sites. The distinction is based on the differences in cation:site interactions between the two classes of sites and the members of the lanthanide series. Lanthanide inhibition of respiration-dependent Ca2+ uptake suggests that the binding site is specific for the calcium ion. Those members of the lanthanide family whose ionic radii are nearer that of Ca2+ are the best inhibitors. The inhibition of respiration-independent Ca2+ binding is much different, indicating non-specific cation absorption. |
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