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Accumulation of short DNA fragments in hydroxyurea treated mouse L-cells |
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| Authors: | Roger F Martin Ian Radford Marshall Pardee |
| Institution: | Biological Research Unit, Cancer Institute, 481 Little Lonsdale Street, Melbourne, 3000, Australia. |
| Abstract: | Treatment of L-cells with hydroxyurea markedly inhibits the incorporation of 3H]thymidine into DNA. The 3H incorporation that persists during hydroxyurea inhibition is largely into 7S DNA chains. The labelled fragments can be chased into higher MW DNA, suggesting that they are intermediates in the replication process. This interpretation concurs with that of earlier reports which describe a similar effect of hydroxyurea on the replication of viral DNA. |
| Keywords: | RBC red blood cells SDS sodium dodecyl sulfate Buffer saline BSA bovine serum albumin 3T3KiMSV 3T3 cells transformed with murine sarcoma virus Kirsten strain TRIS tris-(hydroxymethyl)aminomethane EDTA ethylenediaminetetraacetic acid NET NaCl (150 mM)-EDTA(5 mM)-Tris(50 mM) pH 7 0 containing 0 05% NP-40 Galβ1→3 GalNAcβ1→4[NeurAc2→3]Galβ1→4Glc→ceramide GalNAcβ1→4Galβ1→4Glc→ceramide globoside GalNAcβ1→3Galα1→4Galβ1→4Glc→ceramide paragloboside Galβ1→4GlcNAcβ1→4Galβ1→4Glc→ceramide |
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