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Polyamine levels in normal human serum. Comparison of analytical methods
Authors:Frantisek Bartos  Dagmar Bartos  Donald P. Grettie  Robert A. Campbell  Laurence J. Marton  Ronald G. Smith  G. Doyle Daves
Affiliation:Pediatric Renal Metabolic Laboratory, Department of Pediatrics, University of Oregon Health Sciences Center, Portland, Oregon 97201 USA;Departments of Neurological Surgery and Laboratory Medicine, University of California, San Francisco, California 94143 USA;Department of Chemistry, Oregon Graduate Center Beaverton, Oregon 97005 USA
Abstract:A comparison of levels of the polyamines, putrescine, spermidine and spermine, in individual and pooled normal human serum, as determined by three independent methods, high pressure cation-exchange chromatography (HPCC), radioimmunoassay (RIA) and gas chromatography-mass spectrometry (GC-MS), has been made. Generally good correlations were found for values derived by the three different methods.
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