Polyamine levels in normal human serum. Comparison of analytical methods |
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Authors: | Frantisek Bartos Dagmar Bartos Donald P. Grettie Robert A. Campbell Laurence J. Marton Ronald G. Smith G. Doyle Daves |
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Affiliation: | Pediatric Renal Metabolic Laboratory, Department of Pediatrics, University of Oregon Health Sciences Center, Portland, Oregon 97201 USA;Departments of Neurological Surgery and Laboratory Medicine, University of California, San Francisco, California 94143 USA;Department of Chemistry, Oregon Graduate Center Beaverton, Oregon 97005 USA |
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Abstract: | A comparison of levels of the polyamines, putrescine, spermidine and spermine, in individual and pooled normal human serum, as determined by three independent methods, high pressure cation-exchange chromatography (HPCC), radioimmunoassay (RIA) and gas chromatography-mass spectrometry (GC-MS), has been made. Generally good correlations were found for values derived by the three different methods. |
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