Proteolytic digestion of histones indicates that the nucleosomes of nuclei and of sheared chromatin are similar |
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Authors: | Dawn B Marks Barbara J Keller |
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Institution: | Department of Biochemistry, Temple University School of Medicine Philadelphia, Pa. 19140 USA |
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Abstract: | Calf thymus nuclei were treated with trypsin, chymotrypsin or Pronase, and the rate of digestion of the various histone fractions was determined. The results differed from those obtained by digestion of DNA-free histones with the same set of enzymes but were identical to those obtained by digestion of calf thymus chromatin. Because these enzymes have such different specificities, the results of these digestions indicate that the histone fractions have similar locations in the chromosomal substructures of nuclei and chromatin, that the structure of the nucleosomes which exist within nuclei is not changed markedly when chromatin is isolated from nuclei by a method which involves shearing. |
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