Outer membrane of Salmonella XIV. Reduced transmembrane diffusion rates in porin-deficient mutants |
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Authors: | Hiroshi Nikaido Sun Ah Song Leora Shaltiel Marjatta Nurminen |
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Institution: | 1. Department of Bacteriology and Immunology, University of California, Berkeley, California 94720 USA;2. Central Public Health Laboratory, Helsinki, Finland |
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Abstract: | Rates of diffusion of a β-lactam antibiotic, cephaloridine, across the outer membrane of cells were measured by determining the rates of its hydrolysis by β-lactamases located in the periplasmic space. It was shown that the permeability coefficient of the outer membrane toward cephaloridine decreased to about one-tenth of that in the wild type, in mutant strains deficient in two “porin” proteins, previously shown to produce transmembrane pores in in vitro reconstitution experiments. In contrast, the loss of the 33,000 dalton outer membrane protein did not have any noticeable effect on the permeability coefficient. |
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