Identification of an essential residue of pig heart aconitase |
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Authors: | Paul G. Johnson Abdul Waheed Laudie Jones A.J. Glaid Oscar Gawron |
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Affiliation: | Duquesne University, Pittsburgh, Pennsylvania 15219 USA |
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Abstract: | Treatment of aconitase with phenacyl bromide prior to activation with Fe(II) and reductant results in complete, irreversible enzyme inactivation. Inactivation is due to the alkylation of a cysteine residue at the active site of the enzyme, the inactivation being inhibited by the competitive inhibitor, tricarballylate. Active enzyme is similarly inactivated, citrate affording greater protection than tricarballylate. |
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Keywords: | To whom correspondence should be addressed. |
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