Bestatin,a new specific inhibitor of aminopeptidases,enhances activation of small lymphocytes by concanavalin A |
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Authors: | Masaki Saito Takaaki Aoyagi Hamao Umezawa Yoshitaka Nagai |
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Institution: | 1. Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo-173 Japan;2. Institute of Microbial Chemistry, Shinagawa-ku, Tokyo-141, Japan |
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Abstract: | Bestatin, a new competitive aminopeptidase-inhibitor of dipeptide nature, was shown to enhance markedly activation of peripheral blood lymphocytes by concanavalin A (Con A). More than 40 percent stimulation over the control (the culture with Con A only) was observed at 50 μg/ml of bestatin, and this stimulatory effect was most predominant at an early stage of lymphocyte blasto-genesis by Con A: bestatin had most effect when added to the culture simultaneously with Con A and no appreciable effect when added 44 h after Con A. The effect of bestatin on T lymphocyte activation in vitro is discussed in relation to its in vivo enhancing effect on cell-mediated immunity and a role in lymphocyte blastogenesis of some proteolytic activities possibly located at the cell surface is emphasized. |
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Keywords: | b-TSH bovine thyroid stimulating hormone SDS sodium dodecyl sulfate DOC sodium deoxycholate |
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