Effect of exogenous dolichyl monophosphate on a developmental change in mannosylphosphoryldolichol biosynthesis |
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| Authors: | J.B. Harford C.J. Waechter F.L. Earl |
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| Affiliation: | Department of Biological Chemistry University of Maryland School of Medicine Baltimore, Maryland 21201, USA;Division of Toxicology, Food and Drug Administration Washington, D.C. 20204 USA |
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| Abstract: | The initial rate of mannosylphosphoryldolichol formation by pig brain white matter is 2.9 to 3.3-fold higher in membranes from actively myelinating animals as compared to similar preparations from adults. Exogenous dolichyl monophosphate stimulated mannolipid synthesis in both preparations indicating that the level of the acceptor lipid was rate-limiting. The relative enhancement, however, was higher in membranes from adult animals reducing the ratio of initial rates for young/adult. Exogenous dolichyl monophosphate also stimulated the labeling of a mannosylated oligosaccharide lipid and mannoproteins, including a polypeptide (apparent molecular weight of 100,000) not labeled by gray matter membranes. |
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