Trans-zeatin in culture filtrates of Agrobacterium tumefaciens |
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Authors: | R.W. Kaiss-Chapman R.O. Morris |
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Affiliation: | Department of Agricultural Chemistry Oregon State University Corvallis, Oregon 97331 USA |
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Abstract: | Cytokinin-active bases and nucleosides have been isolated from the culture filtrates of Agrobacterium tumefaciens by trace enrichment onto octadecyl-silica and have been identified by GLC-MS of their permethyl and trimethylsilyl derivatives. Besides the expected 6-(3-methylbut-2-enylamino) purine, the filtrate contained zeatin (85% trans, 15% cis), 2-methylthio-ribosylzeatin and smaller quantities of ribosylzeatin and other cytokinin-active nucleosides. |
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