Changes in membrane fluidity of erythrocytes during cell maturation |
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Authors: | Yoshinao Katsumata Fumio Tanaka Masako Hagihara Kunio Yagi |
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Affiliation: | Institute of Biochemistry, Faculty of Medicine, University of Nagoya, Nagoya 466, Japan |
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Abstract: | The measurements of the fluorescence polarization of perylene embedded in erythrocyte membranes were carried out with normal and reticulocyte-rich blood, and the microviscosity of erythrocyte membranes was calculated from the polarization degree. In intact cells, reticulocyte membranes had a significantly lower microviscosity than normal erythrocyte membranes, while in ghosts no significant difference in membrane microviscosity was observed between reticulocytes and mature erythrocytes. |
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