The chymotryptic phosphopyridoxyl peptide of DOPA decarboxylase from pig kidney |
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| Authors: | F Bossa F Martini D Barra CBorri Voltattorni A Minelli C Turano |
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| Institution: | 1. Centro di Biologia Molecolare del C.N.R., Istituti di Chimica Biologica, Università di Roma e di Perugia Italy;2. Laboratorio di Biochimica Applicata, Università di Camerino Italy |
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| Abstract: | The amino acid sequence of the coenzyme-binding site of DOPA-decarboxylase from pig kidney has been determined. A sample of enzyme was reduced with NABH4, aminoethylated and then digested with chymotrypsin. A single phosphopyridoxyl peptide was isolated and its sequence proved to be: Asn-Phe-Asn-Pro-His-Lys(Pxy)-Trp. Sequence homologies at the active site of various pyridoxalphosphate enzymes, and particularly of the bacterial decarboxylase, are discussed, with some emphasis on the constant presence of a histidine residue adjacent to the phosphopyridoxyl-lysine. |
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