Small-angle X-ray scattering of D-Ribulose-1,5-diphosphate carboxylase from roth (Ag.) in solution |
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Authors: | HHasko Paradies Brigitte Zimmer Günther Werz |
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Abstract: | D-Ribulose-1,5-diphosphate carboxylase from was purified, and the gross dimensions were obtained by means of small-angle X-ray scattering measurements in solution. Dissolved single crystals of this enzyme (called “fraction I protein”) gave the same hydrodynamic parameters as the purified form. The molecular weight was found to be 535,000, and a radius of gyration of Rg = 45.5 Å was determined. The experimental scattering curves revealed a geometrical particle of D-Ribulose-1,5-diphosphate carboxylase with gross dimensions of that of a hollow sphere with outer radius of 56 Å and inner radius of 12 Å. Determinations of the diffusion coefficients lead to the conclusion that the enzyme has a spherical shape of almost uniform density. |
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