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Photolabeling of the adenine nucleotide carrier by 8-azido-ADP
Authors:G. Schäfer  S. Penades
Affiliation:Institut für Klinische Biochemie und Physiologische Chemie Medizinische Hochschule Hannover, Hannover, Germany
Abstract:[14C] 8N3ADP was synthesized from [14C] 8BrADP. It shows atractylate sensitive specific binding to the adeninnucleotide carrier of mitochondria, but is only a weak inhibitor of the translocator. Via nitrene formation uv-irradiation allows covalent labeling of the carrier protein and induces irreversible inhibition of transport. The labeled carrier protein can be isolated; the stoichiometry of labeling is 0.5 moles N3ADP/mole carrier subunit which has a molecularweight of 31000, suggesting a dimer structure of the carrier in situ. Labeling is specific for 8N3ADP; 8N3AMP is inactive as an inhibitor or as photolabel.
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