Photolabeling of the adenine nucleotide carrier by 8-azido-ADP

¹⁴C] 8N₃ADP was synthesized from ¹⁴C] 8BrADP. It shows atractylate sensitive specific binding to the adeninnucleotide carrier of mitochondria, but is only a weak inhibitor of the translocator. Via nitrene formation uv-irradiation allows covalent labeling of the carrier protein and induces irreversible inhibition of transport. The labeled carrier protein can be isolated; the stoichiometry of labeling is 0.5 moles N₃ADP/mole carrier subunit which has a molecularweight of 31000, suggesting a dimer structure of the carrier in situ. Labeling is specific for 8N₃ADP; 8N₃AMP is inactive as an inhibitor or as photolabel.