Purification and characterization of proteins,including procollagen type III,in salt extracts of fetal bovine skin |
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Authors: | Beverly A. Blackwell Howard B. Bensusan |
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Affiliation: | Department of Biochemistry, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA |
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Abstract: | The nature of high-molecular-weight proteins in salt extracts of fetal bovine skin was investigated. A series of DEAE cellulose ion-exchange columns separated the mature collagen from the high molecular weight proteins and also separated the high molecular weight proteins from each other. The following proteins were isolated: (a) a very high molecular weight protein which appears to be aggregated mature collagen; (b) two high molecular weight proteins of slightly faster mobility on SDS polyacrylamide gels, one of which is collagen-like and one of which is not; and (c) a type III procollagen, purer than those previously reported in the literature. These latter three proteins were characterized by amino acid analysis, SDS polyacrylamide gel electrophoretic mobility, collagenase sensitivity, and CNBr peptide patterns from SDS-PAGE. |
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Keywords: | EDTA ethylenediaminetetraacetic acid PMSF phenylmethylsulfonyl fluoride pMB p-(hydroxymercuri)-benzoic acid SDS-PAGE SDS-polyacrylamide gel electrophoresis NSS neutral salt solutions |
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