Protease inhibitors block hormonal activation of adenylate cyclase |
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Authors: | Nancy D Richert Robert J Ryan |
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Institution: | Department of Molecular Medicine, Mayo Medical School and Foundation Rochester, Minnesota 55901 USA |
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Abstract: | To investigate the mechanism of serine protease stimulation of rat ovarian adenylate cyclase, a variety of synthetic protease inhibitors were used. These inhibitors blocked trypsin, chymotrypsin and hCG stimulation of adenylate cyclase in nearly the same manner. The inhibition of hormone stimulated adnylate cyclase could not be explained by a loss of 125I]hCG binding. Cholera toxin and epinephrine stimulation of adenylate cyclase were similarly inhibited, whereas basal and fluoride-stimulated activities were only affected by higher doses of the inhibitors. The results suggest that adenylate cyclase in the ovary may be regulated by membrane protease activity. |
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Keywords: | hCG human chorionic gonadotropin cAMP adenosine 3′:5′ cyclic monophosphate |
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