Isolation of metabolic plasmid DNA from |
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Authors: | James B. Johnston I.C. Gunsalus |
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Affiliation: | Biochemistry Department, University of Illinois, Urbana 61801, USA |
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Abstract: | Metabolic plasmids conferring on the aromatic growth phenotypes naphthalene, Nah+, salicylate, Sal+, or toluate, Tol+, have been isolated as covalently closed circular DNA in 100 μg amounts. Plasmid DNA was banded in CsCl-ethidium bromide density gradients and sedimentation rates measured in sucrose gradients and by analytical centrifuge. The plasmid sizes found, in millions, were /NAH 42, /SAL 43, /TOL 55, 42. Transformation of metabolic plasmid free with the isolated DNA confirmed the respective aromatic pathway gene contents. |
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