Isolation and initial characterization of human basement membrane collagens |
| |
Authors: | Robert L Trelstad Karen R Lawley |
| |
Institution: | Experimental Pathology Laboratory, Department of Pathology, Shriners Burns Institute/Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114 USA |
| |
Abstract: | Neutral solutions of pepsin extracted human collagens derived from glomeruli, kidney, aorta, lung, heart, bowel, spleen, skeletal muscle and skin were subjected to heat gelation at 37°C. Centrifugation of the gel provided two fractions: gelled pellet and non-gelled supernatant. Analysis of these two fractions by gel electrophoresis, molecular sieve and ion exchange chromatography, and amino acid and carbohydrate determinations indicated that the non-gelled supernatant contained a substantial enrichment of basement membrane like collagen. The initial characterization of lung derived basement membrane collagen indicated close similarities with those derived from glomeruli and whole kidney and differences with that obtained from the spleen. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|