Size distribution of poly(A)-containing messencer RNA from free polysomes of HeLa cells |
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| Authors: | William I Murphy Giuseppe Attardi |
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| Institution: | Division of Biology California Institute of Technology Pasadena, California 91125 USA |
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| Abstract: | The sedimentation properties of pulse-labeled and long-term labeled mRNA from highly purified HeLa cell free-polysomes, selected for poly(A) content by two successive passages through poly(T)-cellulose columns, were analyzed under native and denatured conditions. The sedimentation profile of the mRNA on both sodium dodecyl SO4-sucrose gradients and formaldehyde-sucrose gradients showed a broad distribution of components with estimated molecular weights ranging from 2 × 105 to 5.5 × 106 daltons and a weight-average molecular weight of 8.5 × 105 daltons. |
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| Keywords: | mRNA messenger RNA hnRNA heterogeneous nuclear RNA rRNA ribosomal RNA RNP ribonucleoprotein particle |
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