Comparisons of diisopropylfluorophosphate derivatives of chymotrypsin and chymotrypsinogen by phosphorus-31 nuclear magnetic resonance |
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Authors: | Gerald R Reeck TBlaine Nelson Joseph V Paukstelis Delbert D Mueller |
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Institution: | 2. Department of Chemistry Kansas State University Manhattan, Kansas 66506 USA |
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Abstract: | The nature of the differences in the active sites of α-chymotrypsin and chymotrypsinogen has been investigated by phosphorus-31 NMR studies of their diisopropylfluorophosphate derivatives. The phosphorus-31 resonance of the modified zymogen occurs 2 ppm upfield from that for the enzyme. An even greater separation is seen between diisopropylphosphoryl-neo-chymotrypsinogen and -α-chymotrypsin. A plausible interpretation of the chemical shift differences is based on the known structures for α-chymotrypsin, chymotrypsinogen and diisopropylphosphoryl-trypsin. |
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