Singlet oxygen generation in the reaction centers of <Emphasis Type="Italic">Rhodobacter sphaeroides</Emphasis> |
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| Authors: | Adjaci F Uchoa Peter P Knox Rozane Turchielle Nurania Kh Seifullina Mauricio S Baptista |
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| Institution: | 1.Departamento de Bioquímica,Instituto de Química, Universidade de S?o Paulo,Sao Paulo,Brazil;2.Biology Department,M.V. Lomonosov Moscow State University,Moscow,Russia |
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| Abstract: | Singlet oxygen ((1)O(2)) generation in the reaction centers (RCs) of Rhodobacter sphaeroides wild type was characterized by luminescent emission in the near infrared region (time resolved transients and emission spectra) and quantified to have quantum yield of 0.03 +/- 0.005. (1)O(2) emission was measured as a function of temperature, ascorbate, urea and potassium ferricyanide concentrations and as a function of incubation time in H(2)O:D(2)O mixtures. (1)O(2 )was shown to be affected by the RC dynamics and to originate from the reaction of molecular oxygen with two sources of triplets: photoactive dimer formed by singlet-triplet mixing and bacteriopheophytin formed by direct photoexcitation and intersystem crossing. |
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| Keywords: | Photosynthetic bacteria Purple bacteria Photoinhibition Triplets Quantum yield ROS |
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