Cellular Werner phenotypes in mice expressing a putative dominant-negative human WRN gene |
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Authors: | Wang L Ogburn C E Ware C B Ladiges W C Youssoufian H Martin G M Oshima J |
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Institution: | Department of Pathology, University of Washington, Seattle, Washington 98195, USA. |
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Abstract: | Mutations at the Werner helicase locus (WRN) are responsible for the Werner syndrome (WS). WS patients prematurely develop an aged appearance and various age-related disorders. We have generated transgenic mice expressing human WRN with a putative dominant-negative mutation (K577M-WRN). Primary tail fibroblast cultures from K577M-WRN mice showed three characteristics of WS cells: hypersensitivity to 4-nitroquinoline-1-oxide (4NQO), reduced replicative potential, and reduced expression of the endogenous WRN protein. These data suggest that K577M-WRN mice may provide a novel mouse model for the WS. |
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