毕赤酵母表达动物传染病病原基因的研究进展

毕赤酵母表达系统是一种新的真核基因表达系统，由于其有许多突出的优点，如它既有原核生物的特点，又有真核生物的特性，可以对目的蛋白质进行糖基化、二硫键形成等翻译后修饰等特点，越来越受到分子生物学界的重视。已有多种重组蛋白在该系统成功表达，其中许多已被广泛应用于临床诊断治疗或科研工作中。总结了毕赤酵母的表达系统的组成及其在表达动物传染病病原方面的主要研究进展。

Advance of pichia yeast expression system applied in animal infectious disease agent gene

Pichia yeast expression system is a novel eukaryotic expression system which has the characteristics of both prokaryotic and eukaryotic systems. Due to its outstanding virtues, e. g. protein expressed by pichia with some post- translational modifications such as glycosylation and disulfide- bond formation, pichia yeast expression system has attracted more and more attentions from the field of molecular biology. To date,a number of recombinant proteins have been successfully expressed by pichia system,and many have been widely utilized in the clinical disease diagnosis, therapy and research work. This paper summarized the components of pichia yeast expression system and its main research progresses in the expression of animal infectious pathogen.