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Genetic modification of potato development using Ri T-DNA
Authors:G Ooms  A Karp  M M Burrell  D Twell  J Roberts
Institution:(1) Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts., England
Abstract:Summary Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv lsquoDesireersquo with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.
Keywords:Agrobacterium rhizogenes  Potato  Somaclonal variation  Genetic manipulation
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