| 内质网应激及其特有凋亡途径Caspase-12与糖尿病大鼠肾组织固有细胞凋亡之间的关系 |
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| 引用本文： | Cao YP,Hao YM,Liu QJ,Wang J,Li H,Duan HJ. 内质网应激及其特有凋亡途径Caspase-12与糖尿病大鼠肾组织固有细胞凋亡之间的关系[J]. 中国应用生理学杂志, 2011, 27(2): 236-240 |
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| 作者姓名： | Cao YP Hao YM Liu QJ Wang J Li H Duan HJ |
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| 作者单位： | 河北医科大学病理教研室;河北医科大学第二医院内分泌科;邯郸市第一医院急诊科; |
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| 基金项目： | 河北省科技厅资助项目(09276187); 河北省自然科学基金资助项目(c2010000477) |
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| 摘 要： | 目的：检测内质网应激（ERS）标志蛋白在糖尿病大鼠肾组织的表达及其与细胞凋亡之间的关系。方法：单侧肾切除大鼠腹腔注射链脲佐菌素诱发糖尿病,于8周应用免疫组织化学检测GRP78、Caspase-12、PCNA的表达与定位,TUNEL染色检测细胞凋亡部位,流式细胞术检测细胞凋亡程度,并对GRP78、Caspase-12表达水平进行半定量分析,同时观察尿蛋白、BUN、尿肌酐等反应肾功能的相关指标。结果：建模8周,糖尿病组大鼠较对照组肾小球、肾小管凋亡细胞数明显增多,GRP78、Caspase-12表达显著增强。PCNA表达与对照组无明显差异。结论：糖尿病肾损害过程中,ERS被诱导并可能通过激活其特有的凋亡途径Caspase-12引起肾脏细胞过多丢失在糖尿病肾病的发病机制中起重要作用。
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| 关 键 词： | 糖尿病肾病 内质网应激 凋亡 增殖细胞核抗原 |
The relationship between endoplasmic reticulum stress and its particular apoptosis way caspase-12 and apoptosis in renal cortex of diabetic rats |
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| Cao Yan-Ping,Hao Yong-Mei,Liu Qing-Juan,Wang Jian,Li Hang,Duan Hui-Jun. The relationship between endoplasmic reticulum stress and its particular apoptosis way caspase-12 and apoptosis in renal cortex of diabetic rats[J]. Chinese journal of applied physiology, 2011, 27(2): 236-240 |
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| Authors: | Cao Yan-Ping Hao Yong-Mei Liu Qing-Juan Wang Jian Li Hang Duan Hui-Jun |
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| Affiliation: | CAO Yan-ping1,HAO Yong-mei2,LIU Qing-juan1,WANG Jian3,LI Hang1,DUAN Hui-jun1(1.Department of pathology,Hebei Medical University,Shijiazhuang 050017,2.Departerment of Endocrinology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,3.Department of Emergency,The First Hospital,Handan 056002,China) |
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| Abstract: | Objective:To investigate the expressions of 78-kDa glucose-regulated protein(GRP78) and Caspase-12 and their relationship with apoptosis in renal cortex of diabetic rats.Methods:Uninephrectomized Wistar rats were used to induce diabetes by intraperitoneal injection of Streptozotocin(STZ 65mg/kg).After 8 weeks,the expression and distribution of GRP78,Caspase-12,proliferating cell nuclear antigen(PCNA) were examined by immunohistochemistry.Flow cytometry was used to detect the levels of protein of GRP78 and C... |
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| Keywords: | diabetic nephropathy endoplasmic reticulum stress apoptosis proliferating cell nuclear antigen |
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