| 腹腔一次注射法构建百草枯致小鼠肺间质纤维化模型 |
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| 引用本文： | 董雪松,刘盛业,刘伟,刘淑英,刘志.腹腔一次注射法构建百草枯致小鼠肺间质纤维化模型[J].中国实验动物学报,2011,19(4):351-353,I0023,I0024. |
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| 作者姓名： | 董雪松 刘盛业 刘伟 刘淑英 刘志 |
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| 作者单位： | 1. 中国医科大学附属第一医院急诊科,沈阳,110001
2. 中国医科大学95期七年制,沈阳,110001 |
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| 基金项目： | 辽宁省教育厅课题(编号:2008785); 高等学校博士学科点专项科研基金课题(编号:20092104110001) |
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| 摘 要： | 目的建立一种简便易行的百草枯(PQ)致肺间质纤维化动物模型。方法 33只C57BL/6J小鼠随机分为实验组(30只)和对照组(3只)。实验组腹腔一次性注射PQ 10 mg/kg,并于染毒后第2、5、7、14、28天处死小鼠,对照组腹腔注射生理盐水并于第28天处死。观察两组小鼠的一般情况、肺组织大体结构和肺组织病理学改变。结果实验组小鼠在染毒后2 h即出现中毒性改变,肺组织在第28天出现了明显的纤维化改变。结论腹腔一次性注射PQ能简便、可靠的构建出肺纤维化动物模型,可用于进一步研究PQ中毒所致的肺间质纤维化发病机制和治疗方法。
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| 关 键 词： | 肺间质纤维化 百草枯 中毒 动物模型 |
Establishment of a mouse model of pulmonary fibrosis induced by single intraperitoneal injection of paraquat |
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| DONG Xue-song,LIU Sheng-ye,LIU Wei,LIU Shu-ying,LIU Zhi.Establishment of a mouse model of pulmonary fibrosis induced by single intraperitoneal injection of paraquat[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(4):351-353,I0023,I0024. |
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| Authors: | DONG Xue-song LIU Sheng-ye LIU Wei LIU Shu-ying LIU Zhi |
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| Institution: | DONG Xue-song1,LIU Sheng-ye2,LIU Wei1,LIU Shu-ying1,LIU Zhi1(1.Department of Emergency,The First Affiliated Hospital,China Medical University,Shenyang 110001,China,2.Seven-Year Clinical Medicine Program at China Medical University,Shenyang 110001) |
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| Abstract: | Objective To develop an easy and simple method of establishing mouse model of paraquat-induced pulmonary fibrosis.Methods Thirty-three C57BL/6J mice were divided randomly into the experimental group(n=30)and control group(n=3).Paraquat(10 mg/kg) was administrated to the animals of the experimental group by a single intraperitoneal injection.The mice were killed on the day 2,5,7,14 and 28 after paraquat administration.The mice in the control group were treated by an intraperitoneal injection of physiological... |
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| Keywords: | Pulmonary fibrosis Paraquat Intoxication Animal model |
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