| 从ICR小鼠脂肪组织对resistin基因的扩增 |
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| 引用本文： | 鲁帅尧,赵远,唐东红,和占龙,禹文海,邵聪文.从ICR小鼠脂肪组织对resistin基因的扩增[J].中国实验动物学报,2011,19(4):358-360,I0001,I0002. |
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| 作者姓名： | 鲁帅尧 赵远 唐东红 和占龙 禹文海 邵聪文 |
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| 作者单位： | 中国医学科学院北京协和医学院医学生物学研究所,昆明,650118 |
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| 基金项目： | 云南省应用基础研究面上项目(编号:2009ZC185M) |
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| 摘 要： | 目的 PCR扩增ICR小鼠脂肪组织的resistin基因,为进一步在ICR小鼠中开展resistin的相关研究提供必要的基础.方法 在脂肪组织中抽提总RNA,用RT-PCR进行resistin基因的体外扩增,经电泳检测和测序后用DNAMAN软件进行碱基序列分析.结果 抽提的总RNA完整性较好,RT-PCR产物电泳检测获...
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| 关 键 词： | ICR小鼠 脂肪组织 resistin基因 扩增 |
PCR amplification of resistin gene from adipose tissue of ICR mice |
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| LU Shuai-yao,ZHAO Yuan,TANG Dong-hong,HE Zhan-long,YU Wen-hai,SHAO Cong-wen.PCR amplification of resistin gene from adipose tissue of ICR mice[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(4):358-360,I0001,I0002. |
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| Authors: | LU Shuai-yao ZHAO Yuan TANG Dong-hong HE Zhan-long YU Wen-hai SHAO Cong-wen |
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| Institution: | LU Shuai-yao,ZHAO Yuan,TANG Dong-hong,HE Zhan-long,YU Wen-hai,SHAO Cong-wen(Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College,Kunming 650118,China) |
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| Abstract: | Objective To amplify the resistin gene from adipose tissue of ICR mice for further research of its functions.Methods Total RNA was extracted from the adipose tissue for amplification of resistin gene by RT-PCR.The sequence of amplified nucleotide acids was analyzed by DNAMAN software after gel electrophoresis and sequencing.Results The total RNA had good integrity.The expected 363 bp fragment was obtained by RT-PCR.The base peak was uniform in both directions of sequencing.The amplified fragment showed a 93... |
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| Keywords: | ICR mice Adipose tissue Resistin gene Amplification |
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