| 丹参注射液对庆大霉素耳中毒豚鼠耳蜗NOS异构体表达的影响 |
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| 引用本文： | Wang AM,Tang H,Bao DY,Yu L. 丹参注射液对庆大霉素耳中毒豚鼠耳蜗NOS异构体表达的影响[J]. 中国应用生理学杂志, 2011, 27(2): 246-249 |
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| 作者姓名： | Wang AM Tang H Bao DY Yu L |
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| 作者单位： | 辽宁医学院生理学教研室;中国医科大学生理学教研室; |
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| 基金项目： | 辽宁省博士科研启动基金(20031086) |
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| 摘 要： | 目的：研究丹参注射液（SM）对庆大霉素（GM）耳中毒豚鼠耳蜗一氧化氮合酶（NOS）异构体表达的影响,探讨SM对GM耳毒性的防护机制。方法：40只豚鼠随机分成对照组、GM组、SM组和GM＋SM组,应用SABC免疫组织化学方法及显微图像分析技术,观察NOS三型异构体在豚鼠耳蜗的表达;同时结合听脑干反应（ABR）测试,观察用药前后豚鼠听阈的变化。结果：GM＋SM组豚鼠耳蜗诱导型NOS（iNOS/NOSⅡ）表达和ABR阈值均明显低于GM组（P〈0.01）;且iNOS表达变化与ABR阈值改变高度相关（｜r｜〉0.7,P〈0.01）;而各组豚鼠耳蜗神经元型NOS（nNOS/NOSⅠ）和内皮型NOS（eNOS/NOSⅢ）表达均无显著性差异。结论：SM对GM耳中毒后豚鼠耳蜗nNOS和eNOS表达无影响,但可通过抑制GM所致iNOS高表达,以减少NO的过量生成,从而对GM的耳毒性损伤发挥防护作用。
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| 关 键 词： | 丹参注射液 庆大霉素 一氧化氮合酶 耳蜗 |
Effects of salvia miltiorrhiza injection on gentamicin-induced expression of nitric oxide synthase isoforms in guinea pig cochlea |
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| Wang Ai-Mei,Tang Hao,Bao Dong-Yan,Yu Li. Effects of salvia miltiorrhiza injection on gentamicin-induced expression of nitric oxide synthase isoforms in guinea pig cochlea[J]. Chinese journal of applied physiology, 2011, 27(2): 246-249 |
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| Authors: | Wang Ai-Mei Tang Hao Bao Dong-Yan Yu Li |
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| Affiliation: | WANG Ai-mei1,TANG Hao2,BAO Dong-yan1,YU Li1(1.Department of Physiology,Liaoning Medical University,Jinzhou 121001,2.Department of Physiology,China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective:To investigate the effects of salvia miltiorrhiza injection(SM) on gentamicin(GM)-induced expression of nitric o-xide synthase(NOS) isoforms in guinea pig cochlea,and to explore the protective mechanism of SM on GM-induced ototoxicity.Methods:40 guinea pigs were randomly assigned to 4 groups:control group,GM group,SM group and GM plus SM group.Expression of NOS isoforms in the guinea pig cochlea was detected by the SABC method of immunohistochemistry and microscope image analysis technique.Auditor... |
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| Keywords: | salvia miltiorrhiza injection gentamicin nitric oxide synthase cochlea |
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