有氧运动对高蛋氨酸饮食大鼠血浆NO、ET和NO/ET系统的影响

目的：探讨有氧运动对高蛋氨酸饮食大鼠血浆总一氧化氮合成酶（T-NOS）、一氧化氮（NO）、内皮素（ET）和NO/ET系统的影响。方法：雄性Wistar大鼠随机分为正常饮食对照组（对照组）、高蛋氨酸饲料组（高蛋氨酸组）和有氧运动＋高蛋氨酸饮食组（运动干预组）。对照组喂饲普通饲料,高蛋氨酸组和运动干预组喂饲含3%蛋氨酸的高蛋氨酸饲料,运动干预组同时每日同时进行90 min无负重游泳运动,实验共8周。分别测定血浆同型半胱氨酸（Hcy）、ET、NO和T-NOS含量。结果：高蛋氨酸组血浆Hcy含量显著高于对照组达2倍以上（P〈0.01）,T-NOS和NO含量显著降低,ET含量显著升高（P〈0.01）,且NO/ET比值均显著降低（P〈0.05）;与高蛋氨酸组相比,运动干预组血浆Hcy含量显著下降（P〈0.05）,T-NOS,NO含量和NO/ET比值显著升高（P〈0.05）,且与对照组相比上述各项指标无显著差异。结论：高蛋氨酸饮食可诱发大鼠高同型半胱氨酸血症,血浆NO/ET失衡;有氧运动可降低高蛋氨酸饮食大鼠血浆Hcy水平,改善NO/ET失衡,预防高同型半胱氨酸血症。

Effects of training on plasma content of nitric oxide, endothelin and NO/ET system in rats fed with high methionine diet

Objective：To observe the effects of training on plasma content of NO、ET and NO/ET system in rats fed with high methionine diets.Methods：Male Wistar rats were randomly divided into normal diet group（CR）,high methionine diet group（MR） and high methionine diet plus 90min swimming group（T＋MR）.Rats in MR and T＋MR group were fed with the diet which contained 3%of methionine for eight weeks,but rats in control group were fed with ordinary diet.After breeding for eight weeks homocysteine（Hcy） level,T-NOS、NO and ET were measured.Results：Plasma homocysteine of MR increased twofold as compared with that of CR,whereas plasma NO/ET of MR descended significantly（P0.05）,suggesting that hyperhomocysteinemia was induced by the high methionine diet,and plasma NO/ET system was out of balance.Plasma homocysteine content of T＋MR obviously descended,plasma TNOS,NO and NO/ET ascended obviously（P0.05） as compared with that of MR, and the above indicators of group T＋MR were not different as compared with that of CR（P0.05）.Conclusion：Proper training can descend plasma homocysteine level of rats fed with high methionine diet and better balance between NO and ET to prevent developing of hyperhomocysteinemia.