| 微生物多糖合成关键基因挖掘的研究进展 |
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| 引用本文： | 任鄄宝,邹根,张赫男,龚明,吴迪,张忠,杨焱. 微生物多糖合成关键基因挖掘的研究进展[J]. 微生物学通报, 2021, 48(6): 2131-2142 |
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| 作者姓名： | 任鄄宝 邹根 张赫男 龚明 吴迪 张忠 杨焱 |
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| 作者单位： | 1 国家食用菌工程技术研究中心 农业农村部南方食用菌资源利用重点实验室 上海市农业科学院食用菌研究所 上海 201403;2 上海海洋大学食品学院 上海 201306 |
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| 基金项目： | 上海市科委重点研发项目(18391903500)；上海市科技兴农重点攻关项目(沪农科种字[2016]第1-12号)；上海市领军人才项目(2018) |
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| 摘 要： | 随着分子生物学技术的快速发展,功能基因的挖掘在微生物高产多糖合成关键途径研究中变得越来越重要,不断发展的基因挖掘方法和基因组分析工具推进了研究的深入进行.本文主要综述了近年来报道的微生物多糖生物合成途径和多糖合成途径中的关键酶,以及利用多种技术手段和分析软件工具对多糖合成关键基因进行挖掘和验证的相关研究,为微生物多糖合...
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| 关 键 词： | 微生物多糖 多糖合成途径 基因挖掘 功能验证 |
Summary of progress in mining crucial genes involved in microbial polysaccharides synthesis |
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| REN Juanbao,ZOU Gen,ZHANG Henan,GONG Ming,WU Di,ZHANG Zhong,YANG Yan. Summary of progress in mining crucial genes involved in microbial polysaccharides synthesis[J]. Microbiology China, 2021, 48(6): 2131-2142 |
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| Authors: | REN Juanbao ZOU Gen ZHANG Henan GONG Ming WU Di ZHANG Zhong YANG Yan |
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| Affiliation: | 1 National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;2 College of Food Science, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | Profiting from the rapid development of molecular biology technology, functional genomics plays an increasingly important role in analyzing microbial polysaccharide synthesis pathways. Various gene mining methods and genomics analysis tools facilitate the study of genes responsible for microbial polysaccharides synthesis. Here, we summarize the recent studies in biosynthetic pathway of versatile microbial polysaccharide biosynthesis and the key enzymes involved, the techniques and software package useful for gene mining and function verification. This review will provide a reliable technical reference for crucial genes verification in microbial polysaccharide synthesis and feasible solutions in strain engineering with elevating production titer. |
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| Keywords: | microbial polysaccharides polysaccharide synthesis pathway gene mining function verification |
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