Efficient purification of transglutaminase from recombinant Streptomyces platensis at various scales |
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| Authors: | Shie-Jea Lin Yi-Fang Hsieh Pei-Ming Wang Wen-Shen Chu |
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| Affiliation: | (1) Food Industry Research and Development Institute, P.O. Box 246, Hsinchu, 30099, Taiwan, ROC |
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| Abstract: | An efficient system for the fast and efficient purification of transglutaminase from recombinant Streptomyces platensis and expressed in Streptomyces lividans 25-2 is described. Because the purification procedure of this system is flexible, culture broth from laboratory (20 l) and pilot-plant (130 l) fermentations were used to purify the enzyme to electrophoretic homogeneity with high purity (90–95%) and yield (61–77%) within 1 or 2 days. |
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| Keywords: | Fermentation Protein purification Streptomyces lividans Transglutaminase |
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