Translocation of the interleukin-1 receptor-associated kinase-1 (IRAK-1) into the nucleus |
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Authors: | Böl G Kreuzer O J Brigelius-Flohé R |
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Institution: | German Institute of Human Nutrition, D-14558, Potsdam-Rehbrücke, Germany. boel@www.dife.de |
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Abstract: | Interleukin-1 (IL-1) signal transduction involves the recruitment of the IL-1 receptor-associated kinase-1 (IRAK-1). Subsequent signaling finally leads to nuclear translocation of NFkappaB. We here show that the association and autophosphorylation of IRAK-1 was already detectable 30 s after IL-1 stimulation of ECV 304 cells. Significant levels of IRAK-1 accumulated in the nucleus 30 min after IL-1 stimulation shown by Western blot analysis and confocal laser scanning microscopy. Nuclear transfer of IRAK-1 upon IL-1 stimulation was confirmed in the murine T cell line EL-4. This characterizes nuclear localization of IRAK-1 as a possibly essential event in the IL-1 signaling cascade. |
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