Synergistic cellulose hydrolysis can be described in terms of fractal-like kinetics |
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Authors: | Väljamäe Priit Kipper Kalle Pettersson Göran Johansson Gunnar |
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Institution: | Institute of Molecular and Cell Biology, University of Tartu, Vanemuise 46-138, Tartu 51010, Estonia. |
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Abstract: | A fractal-like kinetics model was used to describe the synergistic hydrolysis of bacterial cellulose by Trichoderma reesei cellulases. The synergistic action of intact cellobiohydrolase Cel7A and endoglucanase Cel5A at low enzyme-to-substrate ratios showed an apparent substrate inhibition consistent with a case where two-dimensional (2-D) surface diffusion of the cellobiohydrolase is rate-limiting. The action of Cel7A core and Cel5A was instead consistent with a three-dimensional (3-D) diffusion-based mode of action. The synergistic action of intact Cel7A was far superior to that of the core at a high enzyme-to-substrate ratio, but this effect was gradually reduced at lower enzyme-to-substrate ratios. The apparent fractal kinetics exponent h obtained by nonlinear fit of hydrolysis data to the fractal-like kinetics analogue of a first-order reaction was a useful empirical parameter for assessing the rate retardation and its dependence on the reaction conditions. |
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Keywords: | cellulose cellulase synergistic hydrolysis fractal kinetics substrate inhibition |
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