Characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria. |
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| Authors: | Y Yonezawa H Tokunaga M Ishibashi M Tokunaga |
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| Institution: | Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Korimoto, Japan. |
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| Abstract: | Nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by ATP-agarose and phenyl-5PW column chromatographies. The molecular mass of this enzyme was 15 kDa by time-of-flight mass-spectrometry. This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl. |
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