An application of monoclonal antibodies to identification of leptospires of serogroup icterohaemorrhagiae found in China |
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| Authors: | W H Zhang X F Bai D K Nie Q D Deng |
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| Affiliation: | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing. |
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| Abstract: | For the purpose of improving the procedures of identification of leptospires, a set of 5 monoclonal antibodies with different serological reactivity against serovars of Leptospira interrogans Icterohaemorrhagiae serogroup isolated in China was developed. One hundred and eight strains isolated from epidemic fields in 5 provinces in southern China were distinctly identified into 4 serovars of Icterohaemorrhagiae serogroup by the monoclonal antibody procedure, i.e., 98 isolates were identified as serovar lai, 7 as icterohaemorrhagiae, 2 as copenhageni, and 1 as H2. Factor antiserum procedure was used at the same time as control for typing these strains and an identical result was obtained. |
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