Cloning and characterization of the groE locus from Actinobacillus pleuropneumoniae |
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Authors: | Guy Vézina Marc Sirois Natalie Clairoux Maurice Boissinot |
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Institution: | Département de Chimie-Biologie, Universitédu Québec àTrois-Rivières, Trois-Rivières, Québec G9A 5H7, Canada;Pavillon Charles-Eugène Marchand, UniversitéLaval, Sainte-Foy, Québec G1K 7P4, Canada |
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Abstract: | A 4.4-kb DNA fragment was cloned from Actinobacillus pleuropneumoniae (strain 4074, serotype 1) by genetic complementation with Escherichia coli groES-groEL mutant strains. Sequence analysis of this fragment revealed a purine nucleoside phosphorylase (DeoD)-encoding gene homolog (deoD), heat-shock response-encoding genes for the small (groES) and large subunits (groEL) and a partial open reading frame encoding an alcohol dehydrogenase homolog (adhE). The predicted amino-acid sequence of groES and groEL genes showed extensive sequence identity (80–95%) with other Pasteurellaceae. The gene organization surrounding the groE locus was different from that of Haemophilus infuenzae. When expressed in E. coli, groES-groEL genes were capable of complementing the growth of a λ lytic phage, indicating a structural as well as functional conservation. |
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Keywords: | Chaperone protein Heat shock Swine pathogen Genetic complementation Alcohol dehydrogenase (adhE) Purine nucleoside phosphorylase (deoD) Actinobacillus pleuropneumoniae |
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