Novel sterol metabolic network of Trypanosoma brucei procyclic and bloodstream forms |
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Authors: | Nes Craigen R Singha Ujjal K Liu Jialin Ganapathy Kulothungan Villalta Fernando Waterman Michael R Lepesheva Galina I Chaudhuri Minu Nes W David |
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Institution: | Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA. wdavid.nes@ttu.edu |
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Abstract: | Trypanosoma brucei is the protozoan parasite that causes African trypanosomiasis, a neglected disease of people and animals. Co-metabolite analysis, labelling studies using methyl-2H3]-methionine and substrate/product specificities of the cloned 24-SMT (sterol C24-methyltransferase) and 14-SDM (sterol C14demethylase) from T. brucei afforded an uncommon sterol metabolic network that proceeds from lanosterol and 31-norlanosterol to ETO ergosta-5,7,25(27)-trien-3β-ol], 24-DTO dimethyl ergosta-5,7,25(27)-trienol] and ergosterol ergosta-5,7,22(23)-trienol]. To assess the possible carbon sources of ergosterol biosynthesis, specifically 13C-labelled specimens of lanosterol, acetate, leucine and glucose were administered to T. brucei and the 13C distributions found were in accord with the operation of the acetate-mevalonate pathway, with leucine as an alternative precursor, to ergostenols in either the insect or bloodstream form. In searching for metabolic signatures of procyclic cells, we observed that the 13C-labelling treatments induce fluctuations between the acetyl-CoA (mitochondrial) and sterol (cytosolic) synthetic pathways detected by the progressive increase in 13C-ergosterol production (control<2-(13)C]leucine<2-(13)C]acetate<1-(13)C]glucose) and corresponding depletion of cholesta-5,7,24-trienol. We conclude that anabolic fluxes originating in mitochondrial metabolism constitute a flexible part of sterol synthesis that is further fluctuated in the cytosol, yielding distinct sterol profiles in relation to cell demands on growth. |
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