Comparative characterization of commercially important xylanase enzymes |
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Authors: | Neelima Arora Amit Kumar Banerjee Srilaxmi Mutyala Upadhyayula Suryanarayana Murty |
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Institution: | Bioinformatics Group, Biology Division, Indian Institute of Chemical Technology, Hyderabad-500007, A.P., India |
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Abstract: | Xylanase is an industrially important enzyme having wide range of applications especially in paper industry. It is crucial to gain
an understanding about the structure and functional aspects of various xylanases produced from diverse sources. In this study, a
bioinformatics and molecular modeling approach was adopted to explore properties and structure of xylanases. Physico-chemical
properties were predicted and prediction of motifs, disulfide bridges and secondary structure was performed for functional
characterization. Apart from these analyses, three dimensional structures were constructed and stereo-chemical quality was
evaluated by different structure validation tools. Comparative catalytic site analysis and assessment was performed to extract
information about the important residues. Asn72 was found to be the common residue in the active sites of the proteins {"type":"entrez-protein","attrs":{"text":"P35809","term_id":"549462","term_text":"P35809"}}P35809
and {"type":"entrez-protein","attrs":{"text":"Q12603","term_id":"2494332","term_text":"Q12603"}}Q12603. |
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Keywords: | Xylanase Bioinformatics sequence analysis structure prediction function |
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